DNA
Part:BBa_K1732009:Design
Designed by: Donna Lee Group: iGEM15_Carnegie_Mellon (2015-09-09)
pSB1C3-J23100-B0034-GFP-His-B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 740
Illegal AgeI site found at 209 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence is codon optimized for E.coli
Source
Sequence designed by Cheryl Telmer from Bruchez lab in Carnegie Mellon University.